tep by Step Directions: Enumerate the correct and step by step process on how to use a microscope. Label them from 1 to 5. A. Look through the eyepiece and adjust the iris diaphragm or mirror until the amount of light is satisfactory B. Move the specimen slide around until the slide is in the center of the stage C. Place a specimen slide on the stage. Fasten the specimen slide with the stage clips. D. Turn the revolving nosepiece so that the lowest power objective lens is clicked in position. E. Turn the coarse adjustment and move the fine adjustment knob until the image comes into sharp focus.