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An essential components of media

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Explanation:

1 Nutrients: proteins/peptides/amino-acids.

2 Energy: carbohydrates.

3 Essential metals and minerals: calcium, magnesium, iron, trace metals: phosphates, sulphates etc.

4 Buffering agents: phosphates, acetates etc.

5 Indicators for pH change: phenol red, bromo-cresol purple etc.

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